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p53 Gene Polymorphisms in the Turkish Population
There different RFLPs on the p53 gene were studied in a healthy Turkish population. These RFLPs were located on the exon 4 (CD 47), exon 6 (CD 213) and intron 6 (A-G). CD 47 (C-T) was not present. CD 213 (G) occurred very rarely with a frequency of 0.0114. The frequency of the Int 6 A/G a alteration was found to be 0.70 for G. The heterozygosity rate was 32.72%.

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Authors: Nejat Akar, Ece Akar, kr Cin

Keywords:

  • Year: 1999
  • Number: 4
  • Referance No: 62286

  • Figure 1. Direct detection of p53 Exon 4 CD 47 (C-T) polymorphism with Nci I Restriction enzyme analysis (agarase gel electrophoresis, 2%) Lane 1: X174 Hae III (marker) Lane 2 and 7: Uncut PCR product (296 bp) Lane 3-6: PCR products cut with Nci (254 bp)
  • Figure 2. Direct detection of p53 Intron 6 (A-G) polymorphism with Msp I Restriction enzyme analysis (agarase gel electrophoresis, 2%) Lane 1: X174 Hae III (marker) Lane 2: Uncut PCR product (351 bp) Lane 3-5;9,11: Homozygote for G-G (221 bp). (+/+) Lane 6: Homozygote for A-A (351 bp) (-/-) Lane 7,8,10: Heterozygote individual G and A (351 and 211 bp). (1/-)

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